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Standardized EuroFlow Protocols

All EuroFlow Protocols/standard operating procedures (SOPs) are carefully standardized and validated in multi-center trials.

The following protocols are available:

Pre-analytical protocols

  • EuroFlow target MFI values for Rainbow beads Version 1.10 - 16 november 2023
  • EuroFlow SOP for DxFlex instrument setup and compensation Version 1.2 - 19 July 2023
  • EuroFlow SOP for Sample Preparation Version 1.7 - 1 november 2023
  • EuroFlow SOP for Instrument set-up and compensation Version 1.2.1 - 29 October 2019
  • EuroFlow SOP for instrument set-up and compensation for BD Lyric instrument for the 8-color panels Version 1.8 - 1 July 2019
  • EuroFlow SOP for Instrument set-up and compensation for BD LSRFortessa X-20 Instruments Version 2.1 - 15 October 2018
  • EuroFlow SOP for Instrument set-up and compensation for Navios Instruments Version 1.5.1 - 29 October 2019
  • EuroFlow SOP for bulk lysis in MRD panels Version 1.3 - 25 June 2018
  • EuroFlow guidelines for BM sample collection and transport for MM MRD measurements Version 1.0 - 3 April 2018

Analytical protocols

  • Composition and technical info of EuroFlow panels for PID Version 1.2 - 14 September 2022
  • Composition and technical info of EuroFlow panels for hematological malignancies Version 1.11 - 14 November 2023
  • List of reference and alternative antibodies for hematol malignancies panels Version 1.13 - 13 November 2023
  • Uniform coding of EuroFlow panels Version 1.1 - March 2019

Data analysis

  • Analysis template BCP-ALL MRD panel (Verbeek et al., 2021)

Downloads for the BD Lyric

  • Reagent files (zip) (download and extract)
  • Assay files (zip) (download and extract)
  • Label file (xml)

Reference values per age group

  • Reference values for PB leukocyte populations (EuroFlow PIDOT tube) version 1.0, 24 May 2023
  • Reference values for PB leukocyte populations (EuroFlow LST tube) version 1.0, 24 May 2023
  • Reference values for PB leukocytes and plasma cell populations (EuroFlow IgH Isotype tube) in adults, version 1.0, 24 May 2023
  • Reference values for PB leukocytes and plasma cell populations (EuroFlow IgH Isotype tube) in children, version 1.0, 24 May 2023
  • Reference values for PB CD4+ T cell populations (EuroFlow IMM CD4 tube), version 1.0, 24 May 2023

To download the protocols, please register and log in via this link

Educational videos

BulkLysis

Instrument Setup & Compensation

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